Journal: Neural Regeneration Research

Article Title: Neural stem cell transplantation alleviates functional cognitive deficits in a mouse model of tauopathy

doi: 10.4103/1673-5374.314324

Figure Lengend Snippet: Effects of NSC transplantation on ERK, CREB, and NFκB protein expression levels in the hippocampus of rTG4510 mice. (A) Western blot bands of ERK, pERK, CREB, p-CREB, NFκB, and pNFκB. (B–G) Quantitative results of ERK, pERK, CREB, p-CREB, NFκB, and pNFκB expression. The target protein expression was normalized against the expression of β-actin. Data are expressed as the mean ± SEM ( n = 3/group). * P < 0.05, ** P < 0.01 (one-way analysis of variance, followed by Tukey’s post hoc test). The experiments were repeated three times. CREB: Cyclic adenosine monophosphate-response element-binding protein; ERK: extracellular regulated protein kinase; NFκB: nuclear factor kappa beta; ns: no significance; p-CREB: phosphorylated CREB; PBS: phosphate-buffered saline; pERK: phosphorylated ERK; pNFκB: phosphorylated NFκB; Tg: transgenic mice; WT: wild-type.

Article Snippet: The PVDF membrane was blocked in 2% bovine serum albumin for 2 hours at room temperature and then incubated overnight at 4°C with the following primary antibodies: extracellular regulated protein kinase 1/2 (ERK1/2; rabbit, Cell Signaling, Danvers, MA, USA, Cat# 4695, 1:2000), phosphorylated-ERK1/2 (rabbit, Cell Signaling, Cat# 9101, 1:2000), cyclic adenosine monophosphate-response element-binding protein (CREB; rabbit, Cell Signaling, Cat# 4034, 1:2000), phosphorylated CREB (rabbit, Cell Signaling, Cat# 4095, 1:2000), nuclear factor kappa beta (NFκB; rabbit, Cell Signaling, Cat# 3034, 1:2000), phosphorylated-NFκB (rabbit, Cell Signaling, Cat# 3034, 1:2000), tau (rabbit, 1:1000, Abcam, Cat# ab47579), phosphorylated-paired helical filaments (PHF)-tau pSer202+Thr205 (AT8) (mouse, Thermos Fisher Scientific, Waltham, MA, USA, Cat# MN1020, 1:1000), Ca 2+ /calmodulin-dependent protein kinase II (CamKII; rabbit, Abcam, Cat# ab134041, 1:1000), and phosphorylated-CamKII (rat, Santa Cruz Biotechnology, Cat# Sc-32289, 1:1000).

Techniques: Transplantation Assay, Expressing, Western Blot, Binding Assay, Saline, Transgenic Assay